Fractionation of rat high density lipoprotein by affinity chromatography
نویسندگان
چکیده
منابع مشابه
Analysis of drug interactions with high-density lipoprotein by high-performance affinity chromatography.
Columns containing immobilized lipoproteins were prepared for the analysis of drug interactions with these particles by high-performance affinity chromatography (HPAC). This approach was evaluated by using it to examine the binding of high-density lipoprotein (HDL) to the drugs propranolol and verapamil. HDL was immobilized by the Schiff base method onto silica and gave HPAC columns with reprod...
متن کاملA novel procedure for the separation of high-density lipoprotein subspecies by affinity chromatography
Plasma high-densitjj lipoprotein (HDL) consists of a number of structurally and functionally distinet subspecies. Flotation procedures permit the separation of HDL, and HDL, (density ranges 1.063-1.125 and 1.125-1.21 g/ml, respectively), whilst apolipoprotein (apo) E-rich and apo E-poor subfractions have been obtained by heparin-Sepharose affinity chromatography (Weisgraber & Mahley, 1980). Alt...
متن کاملIdentification and analysis of stereoselective drug interactions with low-density lipoprotein by high-performance affinity chromatography.
Columns containing immobilized low-density lipoprotein (LDL) were prepared for the analysis of drug interactions with this agent by high-performance affinity chromatography (HPAC). R/S-Propranolol was used as a model drug for this study. The LDL columns gave reproducible binding to propranolol over 60 h of continuous use in the presence of pH 7.4 0.067 M potassium phosphate buffer. Experiments ...
متن کاملPreparation of Plasminogen by Affinity Chromatography
Background: Plasminogen is one of the compounds derived from human plasma. Activation of plasminogen produces plasmin. Plasmin is able to lyse fibrinogen, fibrin, and some other human plasma proteins. The aim of the present work was to study the separation of human plasminogen by affinity chromatography using gel lysine Sepharose. Materials and Methods: Normal human plasma was used as the...
متن کاملpreparation of plasminogen by affinity chromatography
background: plasminogen is one of the compounds derived from human plasma. activation of plasminogen produces plasmin. plasmin is able to lyse fibrinogen, fibrin, and some other human plasma proteins. the aim of the present work was to study the separation of human plasminogen by affinity chromatography using gel lysine sepharose. materials and methods: normal human plasma was used as the start...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1978
ISSN: 0014-5793
DOI: 10.1016/0014-5793(78)80010-6